Engineering of cutinases to enable surface hydrolysis and characterization of polyester copolymers.
Copolymers have varying features which determine the physical and other intrinsic properties of the polymer, such as monomer sequence and end-groups. The molecular weight of synthetic copolymers is a major obstacle to their characterization. Current analytical technologies can only give limited information on how chemical-composition distributions within a macromolecular structure correlate with the final properties of the material. Therefore, an in-depth analysis of the polymer microstructure is needed.
In her lecture Eman Abdelraheem discusses the development of degrading enzymes, like cutinases, as analytical tools for the characterization of copolymers. The depolymerization reactions were studied using model homo- and co-polyester polymers. The process was analyzed using LC, LC-MS, SEC-MS to get a deeper understanding of the chemical composition distribution of their co-polyester polymers.
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About the speaker
Eman Abdelraheem received her Master’s degree in Chemistry in Egypt. After working as an assistant lecturer at the university until 2013, she started her PhD studies with Prof. Alexander Dömling at the Institute of Pharmacy at the University of Groningen, the Netherlands. After obtaining her PhD in 2018, she joined the Biocatalysis group at TU Delft working on multi-enzyme cascade reactions under the guidance of Prof. Ulf Hanefeld. Now she is working with Prof. Francesco Mutti and Andrea Gargano on developing different degrading enzymes and using different analytical tools for the characterization of copolymers.